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Arun, T.
- Detection of Novel Variant of Human P85α PI3K with Impaired Insulin Stimulated Lipid Kinase Activity by Pcr and Restriction Digestion
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Authors
Affiliations
1 Department of Biotechnology, PGP College of Arts and Science, Namakkal-637207. Tamilnadu, IN
2 Department of Biotechnology, Bharathidasan University, Trichy-620 024. Tamilnadu, IN
3 Centre for Biotechnology, Muthayammal College of Arts & Science, Rasipuram – 637 408, IN
1 Department of Biotechnology, PGP College of Arts and Science, Namakkal-637207. Tamilnadu, IN
2 Department of Biotechnology, Bharathidasan University, Trichy-620 024. Tamilnadu, IN
3 Centre for Biotechnology, Muthayammal College of Arts & Science, Rasipuram – 637 408, IN
Source
International Journals of Biotechnology and Biochemistry, Vol 8, No 1 (2012), Pagination: 27-34Abstract
Phosphoinositide 3 kinase (PI3K) plays a central part in the mediation of insulin stimulated glucose disposal. The mechanism underlying severe insulin resistance with diabetes mellitus or impaired glucose tolerance in human remain poorly understood but increasing the knowledge of the complexity of intracellular insulin signaling pathways has opened up a large number of candidate genes like PI3K potentially responsible for cases of genetically determined insulin resistance. PI3K - P85α regulatory subunit gDNA was examined in pro band family with syndromes of severe insulin resistance by amplifying and analyzing with special reference to the novel heterozygous mutation gene with a pair of primers flanking the amplification of middle segment of size 894 bp from the full length sequence of human type 2 diabetic gDNA. Mutation in Arg 409 Gly cost the loss of Msp I restriction sites. The amplified product was digested with Msp I enzyme the presence or absence of the site was identified by Agarose gel electrophoresis. Several samples of diabetic patient were tried for PCR amplification of the desired segments. Many didn't amplify. Out of five samples amplify and digested with Msp I two samples were cut by the enzyme denoting the presence of the site and the three samples were not cuted by the enzyme denoting the absence of the site. The impaired PI3K activity due to the mutation of Arg 409 Gly codon of P85 α would contribute the insulin resistance in many type II diabetes mellitus.
References
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- Comparison of the Characteristics of Dental Stem Cells vs. Umbilical Cord Stemcells
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Authors
Affiliations
1 Post Graduate Student, Department of Pedodontics, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
2 Professor and Head of the Department, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
3 Professor, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
4 Senior Lecturer, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
5 Reader, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
1 Post Graduate Student, Department of Pedodontics, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
2 Professor and Head of the Department, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
3 Professor, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
4 Senior Lecturer, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN
5 Reader, Sree Balaji Dental College, BIHER, Pallikarani, Chennai, IN